Table 3 Primer sequences for the amplification of different genes by qRT-PCR.

From: UPLC-Q-TOF/MS, network pharmacology, molecular docking, and experimental validation to explore the mechanisms of toad clothing on rheumatoid arthritis

Gene

Sequence (5’−3’)

GAPDH-F

GGTCCCAGCTTAGGTTCATCA

GAPDH-R

TCTCGTGGTTCACACCCATC

PIK3CA-F

CACTCGTCACCATCAAACATGA

PIK3CA-R

AGGGTTGAAAAAGCCGAAGGT

JAK2-F

GGAATGGCCTGCCTTACAATG

JAK2-R

TGGCTCTATCTGCTTCACAGAAT

EGFR-F

GCCATCTGGGCCAAAGATACC

EGFR-R

GTCTTCGCATGAATAGGCCAAT

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