Fig. 7
Effect of HT on inflammatory cytokines expression and the activation of ERK and NF-κB signaling pathways in M5-stimulated HaCaT cells. (a) Elisa assay was performed to measure the amount of TNF-α, IL-1β, IL-6, IL-17 A, IL-22 and IL-23 release and PCR was performed to measure the mRNA levels of IVL and FLG. (b) Western blot analysis was used to determine the protein levels of p65, p-p65, ERK and p-ERK. Images were cropped, and full-length blots were presented in Supplementary Fig. 7b. (c) Semi-quantification analysis of p-p65 normalized to p65 and (d) p-ERK normalized to ERK. Data were presented as mean ± SD. n = 3 per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001. Ctr: control; HT: hydroxytyrosol; TNF-α: tumor necrosis factor-α; IL: interleukin.
