Table 1 Primer sequences used for gene expression analysis of spermatogenesis stages and related cellular functions.
Target | Cell type / function | Accession number | Sequence (5′–3′) |
|---|---|---|---|
Plzf | Spermatogonia | NM_001033324.3 | F: GCTGCTGTCTCTGTGATGG R: GGGCTGATGGAACATAGGGG |
Tekt1 | Spermatocyte | NM_011569 | F: CACCAGGAAGTCTCAGAGCGAT R: GTAAGCAGGTCATCCGTCTGGT |
Tnp1 | Spermatid | NM_009407.2 | F: TGTGATGCGGCAATGAGC R: CGACTGGGATTTACCCACTC |
Ki67 | Proliferation marker | NM_001081117 | F: GAGGAGAAACGCCAACCAAGAG R: TTTGTCCTCGGTGGCGTTATCC |
Bax | Apoptosis (pro) | NM_017059.2 | F: TTTGCTACAGGGTTTCATCCAG R: GTCCAGTTCATCGCCAATTC |
Bcl2 | Anti-apoptotic | NM_016993.1 | F: GAGAGCGTCAACAGGGAGAT R: ACAGCCAGGAGAAATCAAACA |
β-actin | Housekeeping gene | NM_007393 | F: TCCCTGGAGAAGAGCTACG R: GTAGTTTCGTGGATGCCACA |
