Fig. 1

Cell proliferation assessment based on the optical density (OD) and cell count. Wild-type C. necator H16 cells were cultured under autotrophic conditions. (A) Time course of growth curve based on OD (green) and cell count (blue). Error bars represent standard deviations of four independent biological samples. (B) Comparison of OD values and cell counts at each sampling point (black plots). The data were further divided into two groups: before 36 h (C) and after 36 h (D). Spearman’s correlation coefficients (rs), p-values, and linear regression equations with R2 values (dotted lines) are presented for each group.