Fig. 2
From: microRNA-576-5p ameliorates dexamethasone-induced BMSC injury by suppressing ANXA2
miR-576-5p level was reduced in glucocorticoid treated BMSCs. (A) Classical images of BMSCs with osteocalcin immunofluorescence and alizarin red staining. (B) MTT assay for BMSC proliferation. (C) The flow cytometric analysis for Annexin V-positive and PI negative apoptotic BMSCs. (D-F) qRT-PCR analysis of Bax, Bcl-2 and miR-576-5p relative expression in BMSCs. (G) Western blot analysis of OPG, ANXA2 and RANKL expression in BMSCs and the gray analysis for the western blot results. (**P < 0.01, ***P < 0.001 compared with the Control).
