Table 1 Designed primer sequences.
Gene | Primer | Sequence (5’−3’) | PCR Products |
|---|---|---|---|
Homo GAPDH | Forward | TCAAGAAGGTGGTGAAGCAGG | 115 bp |
Reverse | TCAAAGGTGGAGGAGTGGGT | ||
Homo NLRP3 | Forward | GAAGTGGGGTTCAGATAA | 243 bp |
Reverse | AGACGCAGTCGTGTGTAG | ||
Homo GSDMD | Forward | AAGACGGTCACCATCCCC | 110 bp |
Reverse | AAGGTCCTCTGCTTCTTATCC | ||
Homo CASP1 | Forward | ACAGACAAGGGTGCTGAACAA | 101 bp |
Reverse | TCGGAATAACGGAGTCAATCA | ||
Homo CASP3 | Forward | AGAGGGGATCGTTGTAGAAGTC | 81 bp |
Reverse | ACAGTCCAGTTCTGTACCACG | ||
Homo IL-1β | Forward | CGAATCTCCGACCACCACTA | 186 bp |
Reverse | AGCCTCGTTATCCCATGTGT | ||
Homo IL-18 | Forward | TGACCAAGGAAATCGGCCTC | 72 bp |
Reverse | ATGGTCCGGGGTGCATTATC |
