Fig. 2 | Scientific Reports

Fig. 2

From: Social maintenance masks induced aggression in zebrafish

Fig. 2

Metabolic stability and the nature of mirror-induced aggression. Cumulative time spent in the mirror zone (MZ) in seconds, b. Number of entries to MZ, c. mean speed in mm/s, d. Frequency of transitions, e. Mean number of aggressive acts performed in the 6 min agonistic interaction, f. Cumulative probability distribution of Parallel Index (PI) values for zebrafish under HGRY + M (black) and CNTRL + M (green) conditions, g. Turning index over 20 sample indexes for CNTRL + M (bottom panel in green) and HGRY + M (bottom panel in black) conditions, h. Temporal dynamics of aggression in CNTRL + M and HGRY + M groups. The average number of mirror bites over time is plotted for both groups, with 95% confidence intervals (shaded areas) for each (n = 20/group), i. Scatter density plot of mirror bites for CNTRL + M and HGRY + M groups. Black points indicate individual data, with denser regions reflecting higher frequencies of aggression in the HGRY + M relative to CNTRL + M group. Axes values represent the number of mirror bites in each condition, j. Relationship between valence index (VI) for aggression and response probability. The graph shows response probability as a function of Valence Index for Aggression in HGRY + M (black) and CNTRL + M (green), k. Polar scatter plot of the focal fishes’ (in green and black dots) individual mean resultant vector’s angles α (0˚ to 360˚) combined with corresponding vector lengths R (0 to 1), for each group, l. Box plot of the individual resultant vector’s lengths R projected (R proj) onto the stimulus direction (180˚), m. Heatmap of mirror-induced aggression (MIA) in the HGRY + M group; n. Heatmap for the CNTRL + M. The vertical line divides the stimulus (right/mirror zone) and non-stimulus (left) zones. Box and whisker plots are shown with arithmetic median (middle line), and boxes represent the 1 st and 3rd quartiles. Data shown as mean ± s.e.m. P values are based on non-parametric t test comparing response differences. All asterisks indicate significance (*P < 0.05, **P < 0.01, ***P < 0.001 or ****P < 0.0001), ns: not significant; Mann–Whitney U test.

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