Fig. 5 | Scientific Reports

Fig. 5

From: Time-resolved fluoroimmunoassay improves sensitivity in the diagnosis of primary membranous nephropathy with low phospholipase A2 receptor antibody titer

Fig. 5

Comparison of the identification ability of ELISA and TRFIA. (A–C) The anti-PLA2R antibody levels of PMN patients were significantly higher than that of non-PMN patients. The difference between PMN and non-PMN patients is more pronounced when using the TRFIA method. (D–F) The efficacy of ELISA and TRFIA in identifying PMN and non-PMN patients was analysis by ROC analysis, with AUC reaching 0.712 (ELISA), 0.829 (PLA2R-IgG-TRFIA) and 0.814 (PLA2R-IgG4-TEFIA), respectively. The p value for PLA2R-IgG-ELISA vs PLA2R-IgG-TFRIA was 0.078; the p value for PLA2R-IgG-ELISA vs PLA2R-IgG4-TRFIA was 0.128; the p value for PLA2R-IgG-TRFIA vs PLA2R-IgG4-TRFIA was 0.659. The adjusted cutoff values were as follows: 2.84 RU/ml for ELISA, 0.44 RU/ml for PLA2R-IgG-TRFIA, and 25.07 ng/ml for PLA2R-IgG4-TRFIA. The p-value for comparing PLA2R-IgG-TRFIA with PLA2R-IgG-ELISA was 0.078, while the p-value for comparing PLA2R-IgG4-TRFIA with PLA2R-IgG-ELISA was 0.12. AUC: area under the curve. ***p < 0.001, ****p < 0.0001.

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