Fig. 1

Blue arrows indicate sample collection points. (a) Biopsy samples from 34 livers treated with normothermic machine perfusion (NMP) were collected at three timepoints: pre-SCS or NMP preservation, before the beginning of machine perfusion; post-NMP, after completion of machine perfusion; and reperfusion, after transplantation into the recipient, just before closure. Twenty of the NMP livers were from benchmark criterion donors, 10 donation after brain death (DBD) and 10 donation after cardiac death (DCD). Fourteen NMP livers were from outside benchmark criterion donors, 6 DBD and 8 DCD. (b) Biopsy samples from 13 control livers were collected at two timepoints: baseline (pre), after dissection, while the liver was on the back table in the operating room; and reperfusion, after transplantation into the recipient, before closure. All of the control livers were from benchmark criterion DBD donors. Abbreviations: DBD, donation after brain death; DCD, donation after cardiac death; NMP, normothermic machine perfusion.