Table 1 Primer details used for monoplex and multiplex PCR for the detection of Staphylococcus spp., S. aureus, methicillin resistance (mecA), virulence genes, and selected antimicrobial resistance genes.
Type of PCR | Primers (Gene) | Targeted genes/Organism | Primer sequence (5’– 3’) | Amplicon size(bp) | Annealing temp. (°C) | References |
|---|---|---|---|---|---|---|
Monoplex | tuf | Genus Staphylococcus | F: GAAGAATTATTAGAATTAGT R: GTGATTGAGAATACGTCCTCAAC | 235 | 56 | |
nuc | Staphylococcus aureus | F: GCGATTGATGGTGATACGGTT R: AGCCAAGCCTTGACGAACTAAAGC | 267 | 50 | ||
mecA | Methicillin resistant Staphylococcus aureus | F: TGCTATCCACCCTCAAACAGG R: AACGTTGTAACCACCCCAAGA | 286 | 55 | ||
hla | hla | F-CTGATTACTATCCAAGAAATTCGATTG R-CTTTCCAGCCTACTTTTTTATCAGT | 209 | 63 | ||
sea | sea | F-TTGGAAACGGTTAAAACGAA R-GAACCTTCCCATCAAAAACA | 120 | 63 | ||
icaA | icaA | F-GATTATGTAATGTGCTTGGA R-ACTACTGCTGCGTTAATAAT | 770 | 66 | ||
fnbA | fnbA | F-GCGGAGATCAAAGACAA R-CCATCTATAGCTGTGTGG | 1279 | 58 | ||
aac(3)-iv | Gentamicin resistant gene | F: AGTTGACCCAGGGCTGTCGC R: GTGTGCTGCTGGTCCACAGC | 333 | 58 | ||
sul1 | Sulphonamide resistant gene | F: CGGCGTGGGCTACCTGAACG R: GCCGATCGCGTGAAGTTCCG | 433 | 66 | ||
Multiplex | tetA | Tetracycline resistant gene | F: GGCGGTCTTCTTCATCATGC R: CGGCAGGCAGAGCAAGTAGA | 502 | 63 | |
strA | Streptomycin resistant gene | F: ATGGTGGACCCTAAAACTCT R: CGTCTAGGATCGAGACAAAG | 893 | 63 |
