Fig. 6

Mechanisms of copper removal and interaction with surface functional groups in Halalkalicoccus sp. Dap5 using SEM, TEM, EDS, and FTIR analyses. (a) Control sample and (b) Copper-treated sample: (1) SEM images show an increase in EPS production in the copper-treated sample; (2) TEM images highlight bioaccumulated copper, indicated by the red arrow in the treated sample; (3) Both the EDS spectra and the corresponding tables of elemental weight percentages demonstrate copper biosorption on the cell surface. (c) FTIR spectra identify functional groups involved in copper binding to the cells, where the red graph represents copper-treated cells and the black graph represents control cells.