Fig. 2

Growth curves of (A-C) C. albicans (SC5314), (D-F) C. auris MMC1, and (G-I) C. auris MMC2 isolates in the presence of bio-oil aqueous phase I (BOAP I, from 4.5 to 0.009 mg/mL) of C. nucifera (coconut; left panel, A, D, and G), S. coronata (licuri; middle panel, B, E, and H) and T. catappa (almond; right panel, C, F, and I). Absorbances were measured at 18, 24, 40, and 48 h. Analyses were performed in three independent experiments, with three technical replicates each. The minimum inhibitory concentration (MIC) for each of the three extracts evaluated (coconut, licuri, and almond) was determined as the curve (concentration) where all the absorbances were below the absence of growth “cut off”, defined as three times the average of negative growth controls (dead fungal inoculum). Licuri showed the best performance; additionally, distinct batches of each licuri bio-oil were tested, with similar MIC values for the MMC1 and MMC2 strains.