Fig. 4
Activity of S. coronata (licuri) BOAP I against biofilms of C. auris. (A-B) C. auris isolates (A) MMC1 and (B) MMC2 were seeded on microplates for biofilm formation (1 × 106 cells/mL) and incubated with different concentrations of the licuri bio-oil aqueous phase I (BOAP I, 4.5–0.009 mg/mL), amphotericin B and itraconazole as controls (16–0.0031 μg/mL). (C-D) C. auris mature biofilms of the (C) MMC1 and (D) MMC2 isolates were incubated with distinct concentrations of the licuri bio-oil aqueous phase I (BOAP I, 4.5–0.009 mg/mL) and amphotericin and itraconazole as controls (16–0.0031 μg/mL). The percentage of viability (%) was calculated using the Alamar blue metabolization (Thermo Scientific), dividing the absorbance of each treatment by the absorbance of the positive control for biofilm formation in the absence of drug/treatment (black bars, 100% viability). Results are the average of three independent experiments performed in triplicates. Asterisks denote the one-way ANOVA test using the Dunnet post-test (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001).
