Fig. 1

Foxp3 + Tregs are the major source of IL-10 in lung metastasis formation. (A) Schematic overview of the forced lung metastasis induction using intravenous injection of MC38 cancer cells in Foxp3^RFP; Il10^GFP reporter mice (n ≥ 12 mice per group). (B–D) Frequency of IL-10 + cells in (B) all CD45 + cells, (C) CD3- cells and T cells, and in (D) CD8 + T cells as well as in CD4 + T cells. (E–G) IL-10 expression in (F) Foxp3- IL-10 + cells and (G) Foxp3 + Tregs. (H) General distribution of all IL-10 producing CD45 + cells in healthy lung and lung with metastasis. Data are presented as mean ± SEM. Non-significant (ns): p > 0.05; *p < 0.05; **p ≤ 0.01; ***p < 0.001, as calculated by Mann–Whitney U test.