Fig. 4

miR-204-5P inhibits the expression of IL36γ/PEDF mRNA, exacerbating the inflammatory response of CA-infected hCECs. A Intersection of miRNAs targeting IL36γ and PEDF, as identified from the database screening, with miRNAs associated specifically with fungal keratitis. B Database prediction of the binding sites of miR-204-5p with IL36γ and PEDF mRNA. C PCR detection of miR-204-5p expression. (n = 3). D PCR detection of IL36γ and PEDF expression after adding miR-204-5p. (n = 3). E PCR and ELISA detection of PEDF expression across different groups. (n = 3). F Dual luciferase reporter assay detects the effect of miR-204-5p on PEDF transcription. (n = 3). Data are presented as the mean ± SD; *p < 0.05, **p < 0.01, and ****p < 0.001.