Fig. 2

Confocal images of COCs and oocytes co-incubated with PKH26 labeled EVs for 9 and 21 h during IVM (A) Negative control COCs showed no fluorescence signal in either the CCs or the oocyte. (B) After 9 h of co-incubation, labeled EVs were detected in the transzonal projections and the perivitelline space. Labeled EVs were also present in the CCs (data not shown). (C) After 21 h of co-incubation, labeled EVs were observed in the surrounding CCs. Scale bar: 30 μm. BF: Bright Field; PKH26: EVs labeled with PKH26.