Fig. 4

Representative images of D8 blastocysts derived from oocytes IVM with the presence or absence of EVs derived from small or large follicles. Nuclei counterstained with DAPI are displayed in blue (A), ICM stained with anti-SOX2 antibody is displayed in red (B) and Caspase-positive cells stained with anti-active caspase-3 antibody are displayed in green (C). An overlay is provided in (D). Scale bar: 48 μm.