Table 2 Specific oligonucleotide primers and probes used in real-time RT-PCR Test.

From: A retrospective investigation for the seroprevalence of Chikungunya virus and its co-existence with Dengue virus in Pakistani population, 2014–2015

S.no.

Primer name (genome position) sequence

1.

CHIKV 874 (874–894)

Sequence (5’ to 3’): AAAGGGCAAACTCAGCTTCAC

2.

CHIKV 961 (961–942)

Sequence (5’ to 3’): GCCTGGGCTCATCGTTATTC

3.

CHIKV 899-FAM (899–923)

Sequence (5’ to 3’): CGCTGTGATACAGTGGTTTCGTGTG

4.

CHIKV 6856(6856–6879)

Sequence (5’ to 3’): TCACTCCCTGTTGGACTTGATAGA

5.

CHIKV 6981 (6981–6956)

Sequence (5’ to 3’): TTGACGAACAGAGTTAGGAACATACC

6.

CHIKV 6919-FAM (6919–6941)

Sequence (5’ to 3’): AGGTACGCGCTTCAAGTTCGGCG

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