Fig. 11
From: Comprehensive analysis of MAGE-A10 in pan-cancer and its validation in gastric cancer
Knockdown of MAGEA10 inhibits the proliferation, migration, and invasion of HGC-27 gastric cancer cells in vitro. (A) Expression levels of MAGEA10 in different STAD cell lines. (B) RT-PCR validation of MAGEA10 knockdown efficiency in HGC-27 cells. (C) Western blot validation of MAGEA10 knockdown efficiency in HGC-27 cells. (D) Statistical analysis of grayscale values from three repeated Western blot experiments. (E) CCK-8 assay to analyze the effect of MAGEA10 knockdown on cell proliferation. (F-H) Analysis of the effects of MAGEA10 knockdown on cell proliferation, migration, and invasion through colony formation, cell scratch, and chamber invasion assays. (I) Western blotting to detect the effect of MAGEA10 knockdown on metastasis-related proteins. (J-L) Statistical analysis of results from three independent experiments of colony formation, cell scratch, and chamber invasion assays. Symbols ns, *, **, ***, and **** indicate not significant, P < 0.05, P < 0.01, P < 0.001, and P < 0.0001, respectively.
