Fig. 4

Histological analysis of ovarian tissue sections from mice, stained with hematoxylin and eosin (H&E; 200x), was performed for various groups and compared to the control group. (a): Group I: Normal control; (b): Group II: PCOS group, Was administrative Letrozole (90 µg/kg) and fed HFD; (c): Group III: PCO induced mice treated with MTF(500 mg/kg); (d): Group IV: PCO induced mice treated with MTL(500 mg/kg); (e): Group V: PCO induced mice treated with MVF(500 mg/kg); (f): Group VI: PCO induced mice treated with MVL(500 mg/kg); and (g): Group VII: PCO induced mice treated with Metformin (300 mg/kg b.w). Primary follicles (PF), secondary follicles (SF), corpora lutea (CL). MTL follicles displayed nuclei that were strongly stained (bifid arrow), primordial follicles (dotted arrow), normal stroma (S), atretic follicles (AF) and degenerated follicles (DF).