Fig. 11 | Scientific Reports

Fig. 11

From: Comprehensive characterization of lysosome-dependent cell death reveals prognostic significance and immune landscape in colon adenocarcinoma

Fig. 11

Knockdown of SLC11A1 expression significantly inhibits proliferation and migration of COAD. (A) Protein expression of SLC11A1 detected in NCM460 and SW480 cell lines (n = 3). (B) Quantitative analysis of SLC11A1 protein levels (n = 3). (C,D) Validation of interference efficiency in the siSLC11A1 knockdown model. (E,F) Colony formation assays showing changes in cell proliferation before and after SLC11A1 knockdown (n = 3). (G,H) Transwell assays before and after SLC11A1 interference (n = 3), bar = 50 μm. (I) CCK8 assays (n = 3). (J,K) Protein expression of apoptosis-related proteins before and after SLC11A1 knockdown (n = 3). (L) Quantification of ROS levels before and after SLC11A1 knockdown in SW480 cells (n = 3). Data are presented as mean ± SD, and statistical significance is indicated as *p < 0.05; **p < 0.01; ***p < 0.001.

Back to article page