Fig. 2

ID1 inhibits NFIC and PTEN, while promoting Galectin-3 expression. A: Western blot analysis of ID1, NFIC, PTEN, p-YAP, and Galectin-3 proteins in four groups: NC group, ID1-KD group, NC group, and ID1-OE group; GAPDH as control protein; B: Immunofluorescence detection of ID1 (red fluorescence) and PTEN (green fluorescence) in the NC group, ID1-KD group, NC group, and ID1-OE group; C: RT-qPCR analysis of relative mRNA expression levels of ID1 in the control group, NC group, ID1-KD group, and ID1-OE group; D: CO-IP experimental results diagram. Data are expressed as mean ± standard deviation. N = 3; nsP>0.05; *P<0.05, **P<0.01.