Table 1 Purification table of GBP-RsLEAP30 from E. coli. Protein contents (mg) are expressed per gram of wet biomass and all values are presented as means ± SE from three independent large-scale expressions and purifications.

From: Structural flexibility of a recombinant intrinsically disordered LEA protein from Ramonda serbica

Fraction

Total protein content (mg)

GBP-RsLEAP30 purity (%)a

GBP-RsLEAP30 content (mg)

GBP-RsLEAP30 yield (%)

Lysate

22.0 ± 4.4

61.9 ± 0.8

13.6 ± 2.6

100.0

Flow through (FT)

20.0 ± 3.9

63.4 ± 3.1

13.2 ± 1.9

93.0 ± 5.2

300 mM imidazoleb

2.1 ± 0.6

90.7 ± 0.9

1.9 ± 0.5

13.2 ± 1.7

  1. aThe purity of GBP-RsLEAP30 at each step of purification was estimated by SDS-PAGE. bPooled 300 mM imidazole fraction shown in Fig. 2b.
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