Fig. 1 | Scientific Reports

Fig. 1

From: Identification of a free-N-glycan with difucosylated type-1 lactosamine in cancerous tissue and its validation for cancer diagnosis by urinary testing

Fig. 1

Comparison of normal phase- and reversed phase-HPLC profiles of neutral PA-oligosaccharides obtained from primary lesions of colorectal cancers and their surrounding normal colorectal mucosa. The patterns of PA-oligosaccharides eluted by normal phase-HPLC (A) and by reversed phase-HPLC (B) are shown. Chromatograms of the neutral fraction from colon cancer cells (a, c) and normal colonic cells (b, d) of No. 10 and No. 14 patients (Table S1), respectively. The peaks of gray zones in normal phase-chromatograms were collected, and then subjected to reversed phase-HPLC. Asterisks in (A) indicate GSL glycans containing Lewis A and X. The peaks indicated by a single asterisk were high-mannose type free-N-glycans (M5A’) and the peak indicated by a double asterisk was GSL glycan with A-blood antigen (ALeb) in (B). These GSL glycans were previously reported by Misonou et al10.

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