Fig. 3 | Scientific Reports

Fig. 3

From: Identification of a free-N-glycan with difucosylated type-1 lactosamine in cancerous tissue and its validation for cancer diagnosis by urinary testing

Fig. 3

Two-dimensional mapping of the neutral N-glycan sequentially digested with glycosidase. Elution positions of PA-glycans on normal phase- and reversed phase-HPLC are represented by glucose units (GU) on the y-axis and x-axis, respectively. Closed black circles indicate the position of the neutral difucosylated N-glycan or its products by sequential enzymatic digestions. Closed gray circles show the positions of the authentic compounds. Enzymes used for glycan structural analyses are abbreviated as follows: 2F, α1,2-l-fucosidase; 34F, α1,3/4-l-fucosidase; 3G, β1,3-d-galactosidase; L-Bio, Lacto-N-biosidase. Symbol representations of glycans are as follows; galactose, yellow circle; mannose, green circle; GlcNAc, blue square; fucose, red triangle.

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