Fig. 9 | Scientific Reports

Fig. 9

From: Novel curcumin nanocarrier for targeting drug delivery of mitochondria proves efficacy in in vivo experiments on hepatocellular carcinoma mice models

Fig. 9

Mitochondrial MTT, ROS and GSH analyses. (A) MTT assay. Ex vivo examination of curcumin carriers treatments on cell viability using extracted mitochondria from liver and brain tissues of healthy and tumorized BALB/c mice. All curcumins induced significant declined cell viability in both examined tissues, however, this reduced cell viability is evidently higher in positive tumors rather than tumor negatives. (B) ROS analysis. The effect of the curcumin treatments on the ROS levels of extracted mitochondria from liver and brain tissues in positive tumor BALB/c mice and healthy BALB/c mice. TDC compared with untreated control and FC treated groups significantly (P value < 0.0001) increased the ROS amount in both examined tissues. The amount of ROS was quantified based on the produced DCF fluorescence intensity, utilizing fluorometric analysis. (C) GSH analysis The Glutathione levels of isolated mitochondria from examined tissues in positive tumor and negative tumor samples treated with curcumin carriers. The GSH level only was significantly reduced in mitochondria of liver tissue grafted from TDC treated tumorized mice (P value < 0.001). All graphs are indicate as Mean ± SEM, N = 3 mice per group. Different lowercase letters above the bars indicate significant differences between treatments.

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