Fig. 4
Functional Effects of SREBF2 Silencing and ACOT7 Overexpression in DLBCL Cells A. qPCR analysis of ACOT7 mRNA levels in SU-DHL-4 and Farage cell lines for Oe-NC (Negative control for overexpression) and ACOT7 (ACOT7 overexpression) groups; B. Western blot analysis of ACOT7 protein expression in SU-DHL-4 and Farage cell lines across sh-NC (Scrambled shRNA control), sh-SREBF2 (SREBF2 knockdown), sh-SREBF2 + Oe-NC (SREBF2 knockdown + overexpression control), and sh-SREBF2 + ACOT7 (SREBF2 knockdown + ACOT7 overexpression; rescue group); C. CCK8 assay showing cell proliferation in SU-DHL-4 and Farage cell lines across sh-NC, sh-SREBF2, sh-SREBF2 + Oe-NC, and sh-SREBF2 + ACOT7 groups; D. EdU assay showing cell proliferation in SU-DHL-4 and Farage cell lines across sh-NC, sh-SREBF2, sh-SREBF2 + Oe-NC, and sh-SREBF2 + ACOT7 groups; E. Transwell invasion assay evaluating cell invasion in SU-DHL-4 and Farage cell lines across sh-NC, sh-SREBF2, sh-SREBF2 + Oe-NC, and sh-SREBF2 + ACOT7 groups; F. Flow cytometry analysis of apoptosis levels in SU-DHL-4 and Farage cell lines across sh-NC, sh-SREBF2, sh-SREBF2 + Oe-NC, and sh-SREBF2 + ACOT7 groups. **P < 0.01, ***P < 0.001.
