Fig. 2 | Scientific Reports

Fig. 2

From: Mitofusin 1 in mitochondrial quality control and anti-inflammatory responses in nucleus pulposus cells during disc degeneration

Fig. 2

Expression levels of MFN1 and MFN2 in grade I and Ⅲ disc NPCs. The expression levels of MFN1 and MFN2 in grade I and Ⅲ disc NPCs were analyzed using RT-qPCR (A) and western blotting (B). Control, TNF-α-induced inflammation, anti-inflammation by Vit E, and anti-inflammation by SPN groups were compared. Equal amounts of protein (20 μg per lane) were loaded for western blotting, and the band intensity of MFN1 was semiquantitatively analyzed after normalization to β-actin as the loading control. Data are presented as mean ± standard deviation (n = 3) from paired t-tests. *p < 0.05. MFN: mitofusin, TNF-α: tumor necrosis factor-α, Vit E: vitamin E, SPN: saponin, RT-qPCR: reverse transcription quantitative polymerase chain reaction, NPC: nucleus pulposus cell.

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