Fig. 4
NDP52, MFF, and OPTN expression in grade I and Ⅲ disc NPCs. The expression of NDP52, MFF, and OPTN in grade I disc NPCs was analyzed using RT-qPCR (A) and western blotting (B). In grade Ⅲ disc NPCs, the expression of NDP52, MFF, and OPTN was analyzed using RT-qPCR (A) and western blotting (B). Control, TNF-α-induced inflammation, anti-inflammatory by Vit E, and anti-inflammatory by SPN groups were compared. For western blotting, each band intensity was normalized to that of β-actin. Equal loading of 20 μg total protein per lane was ensured. Data are presented as mean ± standard deviation (n = 3) from paired t-tests. *p < 0.05. NDP52: calcium binding and coiled-coil domain 2, OPTN: optineurim, MFF: mitochondrial fission factor, RT-qPCR: reverse transcription quantitative polymerase chain reaction, TNF-α: tumor necrosis factor-α, MFF: mitochondrial fission factor, NPC: nucleus pulposus cell.
