Fig. 5 | Scientific Reports

Fig. 5

From: Mitofusin 1 in mitochondrial quality control and anti-inflammatory responses in nucleus pulposus cells during disc degeneration

Fig. 5

GAG and proteoglycan expression in grade I and Ⅲ disc NPCs. (A) Sulfate and chondroitin-sulfate GAG expression in grade I and Ⅲ disc NPCs as analyzed using ELISA. (B) Proteoglycan expression levels as analyzed using immunocytochemistry. TNF-α (-) condition served as negative control, with comparison to TNF-α-induced inflammation, anti-inflammation by Vit E, and anti-inflammation by SPN groups. Nonspecific binding and autofluorescence in the negative control were negligible. Data are presented as mean ± standard deviation (n = 3) from paired t-tests. *p < 0.05. GAG: glycosaminoglycan, ELISA: enzyme-linked immunosorbent assay, Vit E: vitamin E, SPN: saponin, TNF-α: tumor necrosis factor-α, NPC: nucleus pulposus cell.

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