Fig. 4
From: A novel D-peptide modulates DCLK1 gelsolin interactions, reducing PDAC tumor growth
Gelsolin and FGF19 as Potential Extracellular DCLK1 Interactors (A) Identification Strategy for DCLK1 NKEBD Interactors: Step 1: BLAST peptide sequence against protein databases; Step 2: Analyze top-ranked proteins; Step 3: Upload and examine 3D structures; Step 4: Validate binding interactions experimentally. (B–E) Co-localization of DCLK1 with Gelsolin and FGF19: (B) DCLK1 and Gelsolin in PDAC; (C) DCLK1 and Gelsolin in CRC; (D) DCLK1 and FGF19 in PDAC; (E) DCLK1 and FGF19 in CRC. (F,G) SPR Binding Analyses: (F) DCLK1 isoform 4 binding to Gelsolin; (G) DCLK1 isoform 4 binding to FGF19. (H) Effects of Peptides on DCLK1-Gelsolin and DCLK1-FGF19 PPI.
