Fig. 1

Single-cell transcriptomic profiling of the multicellular ecosystem of pleomorphic adenoma. (a) Workflow showing the collection and processing of 10 fresh patient samples for scRNA-seq. (b) UMAP plot of 91,545 single cells from scRNA-seq labeled by cell type with different colors. (c) The proportion of different cell populations identified in different tissue type. (d) FeaturePlot showing the expression patterns of key marker genes across different cell subpopulations. (e) Heatmap showing chromosomal copy number variation level in all cell populations, blue indicates deletion and red indicates amplification.