Fig. 3
Subclustering of epithelial cells. (a) Pseudotime trajectory of myoepithelial subsets, divided into three states with group distributions (Top). Heatmap of differential gene expression in malignant and primary cells, with a bar plot showing enriched pathways associated with recurrence (Bottom). (b) Heatmap of the expression regulation by transcription factors, as estimated using SCENIC. (c) Heatmap of the estimated transcription factors module kit using SCENIC. (d) CytoTRACE values showing the distribution of differentiation states for each myoepithelial cell type. (e) Venn diagram showing the number of pseudotime genes with upregulation or downregulation (a cutoff of ≥ 1.2-fold change; orange), marker genes of different myoepithelial cell type(blue), module genes from hdWGCNA (pink), and DEGs in GSE179895(green). The overlapping genes were identified. (f) CytoTRACE values showing the corelation of MIF, TGFBR3 and FN1 with each myoepithelial cell type. (g) Volcano plots showing differential gene expression in MIF- vs MIF + myoepithelial cell (MEC) (left) and TGFBR3- vs TGFBR3 + MEC (right). (h)Hallmark gene set enrichment analysis of upregulated genes in MIF + myoepithelial cells. (i) Representative images of mIHC showing the expression of MYLK (green), CK14 (red), and MIF (pink) in PA sections; scale bar = 100 μm.
