Fig. 4
Subclustering of fibroblast cells. (a) Significantly differentially expressed genes of fibroblast subsets across different tissue types. (b) UMAP plot of 38,541 single cells from fibroblasts used for subclustering. (c) A heatmap of marker genes for each fibroblast type. (d) KEGG pathway enrichment analysis for fibroblast subsets. (e) Pseudotime and CytoTRACE analysis of fibroblast subsets. (Top-left) CytoTRACE analysis showing the predicted cellular differentiation trajectory. (Bottom-left) Pseudotime analysis depicting the progression of cells along the pseudotime axis. (Top-right) CytoTRACE scores across different fibroblast subsets. (Bottom-right) Boxplot comparing CytoTRACE scores among COMP + , LAMA2 + , and SFRP4 + fibroblast subsets. (f) Pseudotime projections of transcriptional changes in gene expression of fibroblast subsets, showing upregulated and downregulated genes based on the manifold. (g) Enrichment analysis showing the upregulation signaling pathways in the development of fibroblast subsets transition.
