Fig. 6
The effects of glucose and GlcN on KLF4, SIRT1, fibronectin, cell cycle profile related proteins, and claudin-1 in HCE-T cells. (A) HCE-T cells (3 × 105 cells/well) were seeded onto 6-well culture plates containing 17.5 mM glucose in culture medium for 24 h, added extra glucose to reach the glucose dosages (17.5, 25, 30, 35, 40, 50 mM) for 24 h, and subsequently treated with vehicle or 10 mM GlcN for 24 h. The cell lysates were subjected to Western blotting analysis using antibodies against the indicated proteins. ACTN was a loading control protein. ns: non-specific band. (B) The protein bands were quantified through pixel density scanning and evaluated using ImageJ. The ratios of protein/ACTN in HCE-T cells were calculated. Bars illustrate the mean ± SD of at least three independent experiments. Student’s t-tests were applied to examine the difference between GlcN treatment group and control group. * p < 0.05 and ** p < 0.01.
