Fig. 7
The effects of glucose and GlcN on the expression of ZO-1 and claudin-1 in HCE-T cells. HCE-T (8 × 104 cells/well) cells cultured on glass coverslips in glucose at 17.5 mM and 50 mM for 48 h and were treated with different GlcN concentrations (1, 5, 10 mM) for 24 h, and the cells were immune-stained with ZO-1 (red), anti-claudin-1 (green), and DAPI (blue). Images were acquired with a Leica Thunder microscope with a 100x objective. Scale bar = 10 μm.
