Fig. 8
The effects of SIRT1 inhibitors in GlcN combined differential glucose on proteins expression and the levels of cytosolic ROS in HCE-T cells. (A-C) HCE-T (3 × 105 cells/well) cells cultured on 6-well plates glass in glucose at 17.5 mM and 50 mM for 48 h and then with 10 µM Ex-527 and 2 µM INZ combined with 8 mM GlcN for 24 h. (A) The cell lysates were immunoblotted with antibodies against the indicated proteins. ACTN served as a loading control protein. ns: non-specific band. (B) Quantified protein bands were derived from pixel density scanning and ImageJ. The ratios of protein/ACTN or phosphorylated protein/total protein were listed. (C) The cells were then subjected to measurement of DCFH-DA intensity for the ROS level, and the median fluorescence intensity of the vehicle was designated as the starting point for M2 gating. Bars characterize the mean ± SD of three independent experiments. # p > 0.05, * p < 0.05, ** p < 0.01, and *** p < 0.001 versus the control group.
