Table 7 qPCR amplification system and PCR conditions.

From: High polyketide synthase gene expression boosts pathogenicity of Klebsiella pneumoniae in the digestive infection

Reagent components

Content (µL)

ddH2O

7.2

2×Taq Pro Universal SYBR qPCR Master Mix

10

PrimerF

0.4

PrimerR

0.4

Template

2

PCR stage

Reaction conditions

Holding stage

95 °C 30 s

Cycling stage (×40)

95 °C 5s, 60 °C 30 s

Melt curve stage

95 °C 15 s, 60 °C 1 min, 95°C15s; 60 °C 15s

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