Fig. 5 | Scientific Reports

Fig. 5

From: A novel colorimetric DNA sensor for rapid detection of feline panleukopenia virus using ZnFe2O4 nanoparticles and TMB modulation

Fig. 5The alternative text for this image may have been generated using AI.

Experimental workflow for evaluating the oxidizing effect of ZFO nanoparticle (NP)-coated slides on TMB substrate. Step 1: Glass slides were coated with different concentrations of ZFO NPs (0.4–0%). Step 2: The coated slides were incubated with TMB substrate for 4 h. Step 3: Then, TMB solution from each slide was collected, transferred to ELISA plates, and mixed with stop solution. Step 4: Optical density (OD) values were measured at 450 nm using an ELISA reader, and results were expressed as the mean of five independent replicates.

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