Fig. 8

Effects of hybridization with varying concentrations (0–1000 ng/µL) of the amplified VP1 target sequence, yielded by real-time PCR, on the reduction of optical density (OD) values generated by TMB oxidation in the presence of 0.3% ZnFe2O4 and 20 picomol/µL oligonucleotide probe. Statistical significance levels are presented for comparisons of the impact of different target fragment hybridization concentrations on TMB oxidation.