Fig. 6 | Scientific Reports

Fig. 6

From: Deubiquitinating enzyme USP42 promotes breast cancer progression by inhibiting JNK/p38-mediated apoptosis

Fig. 6

USP42 downregulation increases breast cancer cells apoptosis via the JNK/p38 signaling pathway. (A, D) Flow cytometry analysis of apoptotic MCF-7 and MDA-MB-231 cells transfected with shUSP42-2# or the negative control, treated with SP600125 (A), SB203580 (D) or DMSO. (B, C) Western blot analysis of cleaved caspase 3, Bcl-2, Bax, p-p38 MAPK, p38 MAPK, JNK and p-JNK expression in MCF-7 and MDA-MB-231 cells transfected with shUSP42-2# or negative control, treated with SP600125 (B), SB203580 (C) or DMSO. β-actin served as an internal reference. (*: p < 0.05, **: p < 0.01, ***: p < 0.001).

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