Fig. 5
BMS-1 plays a crucial role in driving foam cell formation and inducing changes in macrophage phenotypes, particularly affecting CD206 and CD86 expression. (A) The area of Oil Red O staining was quantified in THP-1 cells after 24 h of treatment with or without 50 µg/mL ox-LDL or 1 µM BMS-1. Scale bar: 50 μm. (B) Western blots indicating total CD206, CD86, CD80, CD163, and β-Actin levels in whole cell lysates. (C) Quantification of lipid staining area from panel (A). **** denotes p-value < 0.0001 by Two-way ANOVA (n = 7, > 1000 cells per trial). (D-G) Quantification of CD163, CD80, CD86, and CD206 protein levels from panel (B). **** denotes p-value < 0.0001, ** denotes p-value < 0.01 by Two-way ANOVA (n = 7, > 1000 cells per trial).
