Table 2 Primer sequences used for constructing the TP53RK overexpression vector and truncated vector.
Gene | Sequence (5′ → 3′) |
|---|---|
1-253aa TP53RK-(F) | CCCGGATCCGCCACCATGTACCCATACGATGTTCCA GATTACGCTGCCGCGGCGGCCAGAGCTAC |
1-253aa TP53RK-(R) | GAGGTCCATGGTTGGGTAGTCTAGAGTCGACCCC |
1-100aa TP53RK-(F) | CCCGGATCCGCCACCATGTACCCATACGATGTTCCAG ATTACGCTGCCGCGGCGGCCAGAGCTAC |
1-100aa TP53RK-(R) | CTGGAATATCTGCCCCAGTTTAGTCTAGAGTCGACCCC |
1-171aa TP53RK-(F) | CCCGGATCCGCCACCATGTACCCATACGATGTTCCAGAT TACGCTGCCGCGGCGGCCAGAGCTAC |
1-171aa TP53RK-(R) | ACCTCCAACATGCTCCTGAAATAGTCTAGAGTCGACCCC |
34-253aa TP53RK-(F) | CCCGGATCCGCCACCATGTACCCATACGATGTTCCAGAT TACGCTGCCAGCGGCCTGGAGCTGGTGAAGCAGG |
34-253aa TP53RK-(R) | GAGGTCCATGGTTGGGTAGTCTAGAGTCGACCCC |
78-253aa TP53RK-(F) | CCCGGATCCGCCACCATGTACCCATACGATGTTCCAGATT ACGCTGCCCTTGGCAGACGGCGGACGGTGCAG |
78-253aa TP53RK-(R) | GAGGTCCATGGTTGGGTAGTCTAGAGTCGACCCC |
