Fig. 1 | Scientific Reports

Fig. 1

From: Cold atmospheric plasma restores fulvestrant sensitivity by inhibiting CCND3 in the drug-resistant breast cancer cells

Fig. 1

Cold atmospheric plasma (CAP) inhibits proliferation of fulvestrant-resistant breast cancer cells. (a,b) The drug-sensitive T47D and MCF-7 cancer cells were continuously exposed to fulvestrant with step-wise increases up to 3 and 4 nM for 32 weeks, respectively, to develop drug-resistant T47D/fulR and MCF-7/fulR cells. (c,d) Viability of T47D/fulR and MCF-7/fulR cells was analyzed in fulvestrant concentrations of 0 to 10 µM. The IC₅₀ is denoted by a horizontal line. NC: fulvestrant-sensitive control cells; fulR: fulvestrant-resistant cells. (e,f) Effect of CAP on drug sensitivity of T47D/fulR and MCF-7/fulR cells was analyzed by colony formation assay. Relative colony area is represented as bar graphs next to colony images. (g,h) Effect of CAP on drug sensitivity of T47D/fulR and MCF-7/fulR cells was analyzed by CCK-8 assay. Time points of CAP or fulvestrant treatment are indicated as arrowheads. All experiments were performed at least three times and represented as mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001.

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