Fig. 1 | Scientific Reports

Fig. 1

From: Novel anti-LAG-3 antibody LBL-007 with anti-PD-1 blockade enhances antitumor immunity by promoting T cell-induced apoptosis

Fig. 1

Establishment of a cell co-culture model. (A) Immunofluorescence staining of Jurkat cells after incubation with PHA (2 µg/mL) as indicated. "Removed PHA 48 h" indicates that after the pretreatment of Jurkat cells with PHA for 48 h, which was replaced by medium without PHA, cells were incubated for another 48 h. (B) Immunoblot of Jurkat cell lysates using the indicated antibodies. Cells were stimulated with PHA (2 µg/mL) as indicated. β-actin protein was used as the internal control. Results are representative of three independent experiments. Original blots are presented in Supplementary File 2. (C) Flow cytometry analysis of Jurkat cells after incubation with PHA (2 µg/mL) as indicated. (D) Immunofluorescence staining of MHC-Ⅱ in tumor cells. (E) Immunofluorescence staining of PD-L1 in tumor cells.

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