Fig. 4

Effect of LBL-007 and anti-PD-1 antibodies on T cell-induced apoptosis in tumor cells. (A) Immunoblot of cell lysates from tumor cells co-cultured with Jurkat cells after incubation with the indicated antibodies for 48 h. β-actin protein was used as the internal control. Results are representative of three independent experiments. Original blots are presented in Supplementary File 3. (B) Quantification of cleaved caspase3, cleaved PARP, caspase3 and PARP. The intensity of the bands was quantified using the Tanon Gel Image System. Results are expressed as the ratio of protein to β-actin and to the sample with the lowest expression set as 1 in each type of protein. Results are expressed as the mean ± SD from triplicates. (C) Viability of tumor cells pretreated with Z-VAD-FMK (50 μM) co-cultured with Jurkat cells at a ratio of 1:20 after incubation with the indicated antibodies for 24 h. Results are expressed as the mean ± SD from triplicates. ^*P < 0.05, ^**P < 0.01.