Fig. 5
From: SPP1+ macrophages promote colorectal cancer progression by activating JAK2/STAT3 signaling pathway
In vitro assays to verify the relationship between SPP1+ TAMs and JAK/STAT3 signaling pathway. The group descriptions of Ctrl, Positive, and Negative are the same as in Fig. 4. The DMSO group added DMSO to the medium to culture MC38 cells as a control. WP1066 group added WP1066 in medium; In the Pos + WP14066 group, WP1066-treated MC38 cells were cultured in SPP1+ TAMs conditioned medium. (A,B) WB detection of JAK/STAT3 pathway protein expression levels in the Control, Negative, and Positive groups. Compared with the Control group, ****P < 0.0001, and compared with the negative group, ####P < 0.0001; (C) CCK-8 assay to detect MC38 cell viability in each group; (D) TUNEL staining to detect the apoptosis rate of MC38 cells in each group; (E–G) Immunofluorescence to detect the expression of EMT-related molecules (E-cadherin, N-cadherin, fibronectin) in each group; H–I) WB detection of JAK/STAT3 pathway proteins in the DMSO, WP1066, and Pos + WP1066 groups, Compared with the DMSO group, ****P < 0.0001.
