Fig. 3
Effects of rab25 knockdown and curcumin treatment on the expression of rab25, integrin β1, and talin in HCT 116 cells. (a) siRNA transfection effectively reduced rab25 expression. (b) Cell viability measured by MTT assay after sequential treatment with rab25 siRNA (20 nM) and curcumin (10 µg/mL). (c) Western blot analysis showing that rab25 knockdown transiently increased integrin β1 expression at 24 h, which was not sustained at 48 h. Curcumin treatment led to a marked upregulation of integrin β1 and talin expression at 48 h. (d) Transcriptional levels of rab25, integrin β1 and talin were measured using real-time PCR. Data were analyzed using the Mann–Whitney U test. Each condition was tested in triplicate across three independent experiments. Results are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. C, curcumin; HCT, human colon cancer cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; siRNA, small interfering ribonucleic acid.
