Fig. 3 | Scientific Reports

Fig. 3

From: USP15-dependent modulation of TGF-β/Smad2/3 signaling: implications for chondrocyte matrix degradation and autophagy in osteoarthritis

Fig. 3

Identification of knockdown and overexpression efficiency of USP15 in human OA chondrocytes. (A) GFP fluorescence confirming lentiviral transduction efficiency. Transfection efficiency of each viral transfection group in human OA chondrocytes was observed under inverted fluorescence microscope (×100, bar = 200 μm), GFP intensity reflects viral load, not USP15 expression; (B) Knockdown of USP15 expression in human OA chondrocytes, qRT-PCR, WB detection of USP15 expression; (C) Human OA chondrocytes overexpressing USP15, qRT-PCR, WB detection of USP15 expression. *p < 0.05, Data are representative of three independent experiments using primary chondrocytes from 25 OA patients (n = 25 biological replicates). Values are mean ± SD.

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