Fig. 1

KRAS K117I, K117N, and A146T increase the proliferation rate of NIH3T3 and HCT116 cells in 2D cultures. Shown are representative photomicrographs of the nuclei of proliferating cells (EdU-positive; Cy5) among transfected (a) HCT116, (b) Caco-2, and (c) NIH3T3 cells. All nuclei were counterstained with Hoechst 33,342 (DAPI) after the Click reaction to detect EdU-labelled nuclei. Scale bar: 100 μm. The percent proliferative (d) HCT116, (e) Caco-2, and (f) NIH3T3 cells were estimated by the total number of EdU-positive nuclei divided by the total number of nuclei (pTT: vector only control, WT: wild-type). Shown is a representative of three independent trials performed, each done in triplicate. Statistical analysis was performed by one-way ANOVA and Tukey post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.